ECH-Agarose, 15ml, 45-165µm Particle Size Range, 786-1223, G-BIOSCIENCES (CAT#: STEM-C-3097-LGZ)

ECH-Agarose contains carboxyl functional groups for affinity chromatography through their amine groups. ECH-Agarose is prepared by condensation of Carboxyl Coupling Resin (Cat. # 786-797) with succinic anhydride, resulting in 15-atom hydraulic spacer arm.

The amine group of the ligand was coupled with ECH-Agarose by carbodiimide coupling method. In this coupling method, the free carboxyl groups of the resin react with the amine groups of the ligands, and water-soluble carbodiimides (such as EDC (n-ethyl-n'-(3 -Dimethylaminopropyl) carbodiimide hydrochloride) to form a stable peptide bond.

Size: 15ml
Resin Active group: -COOH groups
Group to be coupled: -NH2 groups
Resin Active group density: 50 µM carboxyl/ ml drained resin.
45-165µm particle size range
Spherical, cross-linked agarose
Resin spacer arm: 15 atom hydrophilic spacer arm.
Coupling conditions: at 4°C- 25°C, pH: 4.5 -6 for 1.5 -24 hrs, Coupling could be done in organic solvents.
No blocking reaction is required after the coupling reaction, saves time
Storage: 2- 8°C

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