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FilterMax F5 Plate Reader, Molecular Devices (CAT#: STEM-SPO-0026-ZJF)

Highlights

Visible absorbance: This reader provides visible absorbance, top-reading fluorescence intensity, and glow luminescence modes in 96- to 384-well plates with wavelength selection options from 340-650 nm.
UV and visible absorbance: This reader provides UV and visible absorbance, top and bottom fluorescence intensity, glow luminescence, FP, and TRF modes in 6- to 1536-well plates with wavelength selection options from 230-750 nm.

Cat Number: STEM-SPO-0026-ZJF

Application: To measure chemical, biological or physical reactions, properties, and analytes within the well of a microplate

Model: FilterMax F5

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Description

The FilterMax F5 Plate Reader is manufactured by Molecular Devices.
The Molecular Devices FilterMax F5 (replacing our old Analyst plate reader) is a versatile instrument that offers detection based on fluorescence, fluorescence polarization, luminescence, absorbance, and time-resolved FRET. It will operate in 96 and 384 well plate format, and has installed filters for fluorescence of 370, 485, and 535 nm for excitation wavelengths, and 535, 595, and 625 nm options for emission.
This unit comes certified with 90-day warranty (US/Canada). A computer loaded with software is included.

Specification

Condition: Certified pre-owned
Read modes:
Absorbance
Fluorescence (top/bottom read)
Luminescence (top read)
Time-Resolved Fluorescence (top read)
Fluorescence Polarization (top read)
Wavelength ranges:
Abs: 230 - 650 nm
FL Ex: 230 - 650 nm
FL Em: 390 - 700 nm
Lumi: 400 - 700 nm
TRF: 340 - 700 nm
FP Ex: 300 - 650 nm
FP Em: 390 - 700 nm
Wavelength selection: Filters
Absorbance photometric accuracy/linearity:
<±0.75% and ±0.010 OD from 0.1 OD to 3.0 OD (>399 nm)
<±0.75% and ±0.010 OD from 0.1 OD to 2.5 OD (<400 nm)
Absorbance photometric precision/repeatability:
<±0.5% and ±0.005 OD at 2.0 OD (400 nm to 650 nm)
<±0.5% and ±0.005 OD at 1.0 OD (230 nm to 399 nm)
Sensitivity top read optimized:
FL: 0.3 fmol/well in 200 μL fluorescein 96 well (1.5 pM) , 0.1 fmol/well in 75 μL fluorescein 384 well (1.3 pM)
Lumi: 2 fmol/well in 200 μL ATP 96 well (10 pM), 0.75 fmol/well in 50 μL ATP 384 well (15 pM)
TRF: 30 amol/well in 200 μL Europium 96 well (0.15 pM), 15 amol/well in 100 μL Europium 384 well (0.15 pM)
FP: 1 mP at 10 nM fluorescein 96 well, 1.5 mP at 10 nM fluorescein 384 well
Light source(s):
High power LEDs
Deuterium lamp
Detector(s):
Silicon photodiode
Photomutiplier tube
Read types (endpoint, kinetic, etc):
Endpoint
Kinetic
Well scan
Read times:
Abs: 27 seconds
FL: 29 seconds
Lumi: 28 seconds
FP: 54 seconds
Plate shaking:
Linear
Orbital

Features

Visible absorbance: This reader provides visible absorbance, top-reading fluorescence intensity, and glow luminescence modes in 96- to 384-well plates with wavelength selection options from 340-650 nm.
UV and visible absorbance: This reader provides UV and visible absorbance, top and bottom fluorescence intensity, glow luminescence, FP, and TRF modes in 6- to 1536-well plates with wavelength selection options from 230-750 nm.

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