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ForteBio Octet Red96e System, ForteBio (CAT#: STEM-CTEPO-0060-ZJF)

Highlights

High-quality kinetic screening and affinity characterization
Microfluidics-free Dip and Read™ format reduces assay time and maintenance cost
Eight parallel, independent channels for maximum speed and flexibility

Cat Number: STEM-CTEPO-0060-ZJF

Application: Detect a diverse range of biomolecules from small molecules to proteins to mammalian cells

Model: Octet Red96e

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Description

The ForteBio Octet Red96e System is manufactured by ForteBio.
The Octet RED96e system directly measures the presence of specific proteins and other molecules in solution with minimal interference from complex matrices. Accurate and reproducible concentrations can be determined in as little as two minutes per sample or 32 minutes for a whole plate using a simple, one-step assay (Figure 1). High sensitivity in quantitation can be achieved to sub-ng/ml levels with 2-step and 3-step assay formats, allowing automated measurement of contaminants such as host cell proteins and residual protein A faster and more precisely than ELISA. Process economics can be improved further by regenerating and re-using the biosensors.
This unit will be certified and comes with a 90-day warranty. A computer preloaded with software is included.

Specification

Condition: Certified pre-owned
Detection technology: Bio-Layer Interferometry (BLI)
Biosensor type: Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking in the sensor tray
Information provided:
Yes/No binding
Kinetic and affinity analysis (kobs, ka, kd, KD)
Specific and selective detection of molecules, even in crude samples
Relative and absolute quantitation of specific proteins in crude matrices or purified samples
Data presentation:
Plots displaying real-time kinetic binding sensorgrams, fitted result plots, and residuals of fits
Concentration data analysis including alibration curves and output of tabulated concentration data
Tabulated kinetic data
Epitope binning and cross-blocking matrices and trace overlays
Sample types: Proteins, antibodies, peptides, DNA, RNA, liposomes, bacterial cells, viruses, mammalian cells, small molecules in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates
Number of spectrometers: 8
Maximum simultaneous reads: 8
Data collection rate: 2, 5, or 10 Hz
Sample position and format: 1 standard 96-well, black, flat bottom microplate
Sample volume: 180–220 μL/well, non-destructive testing
Orbital flow capacity: Static or 100–1500 rpm
Analysis temperature range: 15–40°C, 1°C increments
Dimensions (H x W x D): 19.5 in x 22 in x 18.2 in (49 cm x 56 cm x 46 cm)
Weight: 72 lb (32.7 Kg)
Electrical requirements: Mains: 100–120/200–240 VAC, 50/60 Hz, 4 A max
Power consumption: 200 W (300 W peak)
Warranty: 90-day warranty

Features

High-quality kinetic screening and affinity characterization
Microfluidics-free Dip and Read™ format reduces assay time and maintenance cost
Eight parallel, independent channels for maximum speed and flexibility
Versatility to detect anything from small molecules to mammalian cells
Non-destructive sampling allows full sample recovery
Up to 12 hours of unattended run time
Sample plate cooling for temperature sensitive proteins
Perfectly suited to operate in GxP-regulated environments

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