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Glutathione-S-Transferase (GST) Tag Protein Purification by GST Magnetic Beads (CAT#: STEM-MB-1310-LGZ)

Introduction

Magnetic bead purification technology has significant advantages over traditional protein and nucleic acid purification techniques. Rapid purification of proteins and antibodies is simple with coated magnetic beads. Save time and money by replacing nucleic acid-based purification columns with silica-coated magnetic bead technology.




Principle

GST MagBeads are a series of magnetic particles with an average diameter of 20-75 μm, coupled with reduced glutathione, and are an ideal medium for quick and easy purification of GST fusion proteins.

Applications

For the purification of Glutathione-S-Transferase (GST) tag protein.

Procedure

1. Buffer preparation.
2. Sample Preparation
3. Bead preparation.
4. Purification of target protein.

Materials

• Washing Buffer
• Elution Buffer
• Neutralization Buffer
• Storage Buffer
• Magnetic Stand or magnet
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