HEK293/Human Glypican-3 (GPC3) Stable Cell Line, ACROBiosystems (CAT#: STEM-CT-0714-ZJF)

Overexpression stable cell lines are genetically engineered to consistently produce high levels of a specific gene. This process involves enhancing the gene's expression and introducing it into cells through techniques like lentiviral transduction or electroporation, a strategy known as ‘Gain-of-Function’. As a result, these cells present the targeted antigen on their membrane surface, a trait that remains stable over numerous cell divisions. This method is a long-established approach in molecular biology for exploring gene functionality and remains crucial in biological research. These stable cell lines are invaluable tools for gene research, providing a robust foundation for the development of new pharmaceuticals.

Growth Properties: Adherent
Selection Marker: Puromycin (2 μg/mL)
Culture Medium: DMEM medium + 10% FBS
Freeze Medium: Serum-free cell cryopreservation medium
Quantity: 1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
Storage: Frozen in liquid nitrogen.

Exploring Gene Function: Useful in studying various gene functionalities, including immunity, cell destruction, proliferation, and more.
Antibody Drug Screening: Effective for testing antibody drug activities, including cellular level binding and blocking.
CAR Therapy Cytotoxicity Assessment: Suitable for evaluating the cytotoxic impacts of CAR therapies.

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