HisTALON™ Superflow Cartridge Purification Kit, 5 x 5 mL Cartridges, 635681, Takara Bio Inc (CAT#: STEM-C-3834-LGZ)

HisTALON Superflow Cartridges are FPLC cartridges pre-packed with TALON Superflow resin, which can withstand a flow rate of 5-20 cm per minute. This resin combines Superflow 6, a rigid, highly porous agarose derivative, with TALON. Columns prepacked with resin can be used to efficiently purify His-tagged proteins from total soluble protein extracts of bacterial, mammalian, or baculovirus-infected cells.

TALON Metal Affinity Resin is a durable immobilized metal affinity chromatography (IMAC) resin with remarkable affinity and specificity for his-tagged proteins. TALON resins are compatible with all commonly used IMAC reagents and allow protein purification under native or denaturing conditions.

TALON metal affinity resins use cobalt ions to purify recombinant his-tagged proteins. TALON is a cobalt-containing tetradentate chelator that is specific for his-tagged proteins. The TALON reaction core contains cobalt, which has strict requirements on the spatial positioning of histidine. Only adjacent histidines or specially positioned histidines can combine with cobalt in the reaction core. In nickel-based resins (ie, Ni-NTA resins), these space requirements are less stringent. Therefore, nickel-based resins are also capable of binding histidines located at positions other than the his-tag of proteins.

TALON Superflow Resin is designed for fast and efficient purification of his-tagged proteins at high flow rates and medium pressures (up to 150 psi), ideal for FPLC applications.

Size: 5 x 5 mL Cartridges

Easy-to-use pre-packaged 1ml and 5ml cartridges
Maximize the yield of bioactive protein
High purity, high yield

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