Immobilized Protein G Resin, 5ml Resin, 786-284, G-BIOSCIENCES (CAT#: STEM-C-3245-LGZ)

Immobilized protein G is used to bind the constant domains of immunoglobulin (Ig) molecules. It is an ideal protein for simple one-step purification of immunoglobulin classes, subclasses and fragments from biological fluids and cell culture media. Protein G is a modified streptococcal group G so that it does not bind albumin. Protein G is coupled to 4% highly cross-linked agarose beads via a proprietary conjugation method, providing high coupling efficiency and minimal protein G leaching for Ig. Our immobilized protein G has a high binding capacity (>20mg sheep IgG/ml resin).

Protein G can be used to purify mammalian monoclonal and polyclonal IgGs that bind poorly to Protein A. Protein G has a stronger affinity than protein A for most mammalian IgGs, especially for certain subclasses, including human IgG3, mouse IgG1, and rat IgG2a. Unlike protein A, protein G does not bind human IgM, IgD and IgA.

Immobilized Protein G 0.2ml spin column kit is suitable for small-scale affinity purification of antibodies from various samples. Each 0.2ml chromatographic column can purify 0.1-1mg IgG from 25-500μl serum or other samples.

Immobilized Protein G 1ml Spin Column Kit is suitable for small-scale affinity purification of antibodies from various samples. Each 1ml chromatographic column can purify 1-13mg IgG from 0.5-2ml serum or other samples.

Can also process up to 96 samples in a 96-well spin plate format.
Specific binding and elution buffers can also be used.

Size: 5ml resin
High binding capacity: 38mg human IgG/ml resin; >20mg sheep IgG/ml resin
Ligand: Recombinant Streptococcal Protein G lacking the albumin-binding domain produced in E. coli
Bead size: 50-165μm
Bead Structure: 4% highly cross-linked agarose
Maximum flow rate 1,000cm/hr
Maximum pressure 300kPa

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