Inertsil HILIC Analytical Columns manufactured by GL Sciences Inc. are alternatives to reversed phase chromatography for highly polar compounds. The column chemically bonded with diol group provides excellent peak shape for neutral and basic compounds. In HILIC mode, generally when organic solvent concentration is raised, it shows a strong retention. Moreover, it can get more stable analysis by adding basic solvent to the eluate, such as ammonium acetate.