InertSphere Sugar-2 manufactured by GL Sciences Inc. is a column for analysis sugar, it packed with Ca2+ loaded resin. The column mainly is used for SEC (Size Exclusion Chromatography) mode, the elution order is from big molecular weight to small. At the same time, the ligand exchange mode employs ion interaction between metal ions and hydroxyl groups form a complex with the counter ion. Especially this mode is effective for analysis sugar-alcohol. 100 % water can be used as eluent, there is no need to prepare mix solvent.