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Inter simple sequence repeat (ISSR)-PCR is a technique that involves the use of microsatellite sequences as primers in a polymerase chain reaction to generate multi-locus markers.<br />The main advantage of ISSRs is that no sequence data for primer construction are needed. Because the analytical procedures include PCR, only low quantities of template DNA are required.<br />It is a simple and quick method that combines most of the advantages of microsatellites (SSRs) and amplified fragment length polymorphism (AFLP) with the universality of RAPD.