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Inter simple sequence repeat (ISSR)-PCR is a technique that involves the use of microsatellite sequences as primers in a polymerase chain reaction to generate multi-locus markers.
The main advantage of ISSRs is that no sequence data for primer construction are needed. Because the analytical procedures include PCR, only low quantities of template DNA are required.
It is a simple and quick method that combines most of the advantages of microsatellites (SSRs) and amplified fragment length polymorphism (AFLP) with the universality of RAPD.