Investigation of the mobility of Rab7 and LAMP1 on the phagosomes in macrophages by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0065-WXH)

Introduction

During phagosome maturation, the late endosomal marker Rab7 and the lysosomal marker LAMP1 localize to the phagosomes.
Rab5 is associated with early phagosomes followed by recruitment of its effector proteins, EEA1 and Class III phosphatidylinositol 3-kinase. Rab7 appears on the phagosome membrane after Rab5 dissociation and resides there during phagosome maturation. Rab7 regulates the transportation and fusion of late endosomes and lysosomes. Lysosomal-associated membrane protein 1 (LAMP1) and LAMP2, the major components of lysosomes, accumulate in phagolysosomes via the fusion of lysosomes with the phagosome.