Kinetic analysis of drug transport in multidrug resistant cells by Quench-Flow method (CAT#: STEM-AC-0048-WXH)

Introduction

Multidrug resistance (MDR) is a phenomenon whereby cells confer drug resistance to structurally and functionally unrelated compounds.
One major feature of multidrug resistance is the reduced cellular level of drugs maintained by MDR cells.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

The principle is to mix the two solutions and then to observe a change in spectroscopic properties of the mixture at different place along the reaction tube during the flow. In the continuous mode of the quenched-flow method, the observation chamber is replaced by a second mixer in which the quenching agent arrives.

Applications

Used to determine fast reaction rates or single turnover rates of enzymatic reactions and to isolate reaction intermediates.

Procedure

Small volumes of solutions are driven through a high efficiency mixer and flow into a delay (or ageing) loop. After a set time, the reaction is stopped (or quenched) by the addition of a chemical quench solution.

Materials

Quench flow

Other recommended products

Study of The Kinetics of the Redox Reactions of Ubiquinone by Quench-Flow method (CAT#: STEM-AC-0044-WXH)
Kinetic analysis of polymerases by Quench-Flow method (CAT#: STEM-AC-0034-WXH)
Study of Kinetics of the double-stranded (ds) DNA unwinding by Quench-Flow method (CAT#: STEM-AC-0033-WXH)
Analysis of protein dynamics by Quench-Flow method (CAT#: STEM-AC-0042-WXH)
Study of protein phosphorylation by Quench-Flow method (CAT#: STEM-AC-0049-WXH)
Study of calcium release from isolated sarcoplasmic reticulum by Quench-Flow method (CAT#: STEM-AC-0045-WXH)
Study of cryoenzymology by Quench-Flow method (CAT#: STEM-AC-0043-WXH)
Kinetic analysis of adenosine triphosphatases (ATPases) by Quench-Flow method (CAT#: STEM-AC-0035-WXH)