Kinetic analysis of polymerases by Quench-Flow method (CAT#: STEM-AC-0034-WXH)

The principle is to mix the two solutions and then to observe a change in spectroscopic properties of the mixture at different place along the reaction tube during the flow. In the continuous mode of the quenched-flow method, the observation chamber is replaced by a second mixer in which the quenching agent arrives.

Used to determine fast reaction rates or single turnover rates of enzymatic reactions and to isolate reaction intermediates.

Small volumes of solutions are driven through a high efficiency mixer and flow into a delay (or ageing) loop. After a set time, the reaction is stopped (or quenched) by the addition of a chemical quench solution.

Quench flow