MacroCap SP, 25 mL, Cytiva (CAT#: STEM-C-1578-LC)

MacroCap SP maufactured by Cytiva is a cation exchanger designed for the purification of large biomolecules such as polyethylene glycol (PEG)-modified proteins (i.e., PEGylated proteins) that are intended for use as biopharmaceuticals. It allows separation of mono- from oligo- and non-PEGylated proteins with high selectivity under high load conditions. MacroCap SP is based on acrylamide-dextran copolymer resins with mass transfer properties suitable for large biomolecules. The base matrix is highly porous, which offers high available surface area for adsorption of PEGylated proteins. The large pore size also makes MacroCap SP suitable for binding other large proteins, such as IgM. MacroCap SP is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. MacroCap SP meets industrial demands with security of supply and comprehensive technical and regulatory support.

Chromatography technique: Ion Exchange Chromatography
BioProcess resin: Yes
Ligand: SO3-
Particle size, d50V1: ~50 µm
Ion Exchanger Type: Strong cation exchanger
Matrix: Acrylamide-dextran copolymer
Ionic Capacity: 0.10-0.13 mmol H+/ml medium
pH stability, operational2: 4-11
pH stability, CIP3: 2-13
Flow rate: < 120 cm/h in BPG 300, 20-cm bed height or 70 cm/h in BPG 300, 30-cm bed height using process buffers with the same viscosity as water at v 3 bar (0.3 MPa)
Storage: 4 to 30°C 0.2 M Sodium Acetate in 20% Ethanol
Chemical stability: Commonly used aqueous buffers, 0.5 M NaOH, 0.1 M citric acid, 25% ethanol, 30% propanol, 30% methanol, 50% ethylene glycol, 1% Tween-20, and 1% SDS.
Pack size: 25 mL

High purity and yield of PEGylated proteins at high sample loads
Highly porous base matrix makes extensive surface area available for adsorption of large molecules

Capto DEAE, 10 L, Cytiva (CAT#: STEM-C-1547-LC)