MagINDEX™ rProtein A Magnetic Agarose, Settled Resin, 10 mL, 11-0271-010, Marvelgent biosciences (CAT#: STEM-C-3701-LGZ)

Magnetic Sepharose is an affinity chromatography medium designed for fast, simple, one-step purification of immunoglobulins, especially the IgG isotype, and fusion proteins containing the Fc region.

Proprietary MagIndex™ technology facilitates encapsulation of superparamagnetic cores in a mechanically stable agarose matrix, which is optimal absorbance for high-yield protein purification with minimal background.

Superparamagnetic cores have a very fast magnetic response in a magnetic field, but have no memory when the magnetic force is removed. The agarose-based encapsulation provides a strong shield that protects the core well in a wide range of reagents commonly used in protein purification. In addition, as an ideal protein purification matrix, agarose matrix can achieve high-purity and high-capacity purification of antibodies and Ig fragments in high-throughput screening and various preparation scales.

Superparamagnetic core with fast response to magnetic field
Low non-specific binding, detection accuracy
High density of protein A ligands for optimal antibody and Ig fragment yields
High stability in common buffer systems and detergents

Protein A is a bacterial cell wall protein isolated from Staphylococcus aureus that binds to the Fc region of most immunoglobulins through interaction with the heavy chain. Native protein A contains five high-affinity IgG-binding domains and many domains with unknown functions.

Recombinant protein A is a genetically engineered protein that contains only 5 high-affinity IgG binding domains. Non-essential domains were removed to minimize non-specific binding. Recombinant protein A is produced in E. coli and it binds immunoglobulins with essentially the same affinity as native protein A. Protein A can have a high affinity with the Fc region of IgG of various species such as human and rabbit.

Size: 10 mL
Ligand: Recombinant protein A containing only Ig binding domains
Binding capacity: >10 mg human IgG/mL medium
Matrix: Cross-linked agarose beaded particles containing the superparamagnetic core, supplied as a 20% slurry
Particle size: 30 μm - 100 μm
pH stability: Long term: pH 2 - 9; Short term: pH 2 - 11
Storage temperature: 2 - 8°C
Storage buffer: PBS containing 0.01% Tween-20 and 0.02% NaN3

Large volume, > 10 mg human IgG / mL of settled resins
High selectivity, high purity

Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 500mm Length, 7bar, GB-10-500-1TE, G-BIOSCIENCES (CAT#-STEM-C-3386-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 600mm Length, 7bar, GB-10-600-1TE, G-BIOSCIENCES (CAT#-STEM-C-3387-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 700mm Length, 7bar, GB-10-700-1TE, G-BIOSCIENCES (CAT#-STEM-C-3388-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 800mm Length, 7bar, GB-10-800-1TE, G-BIOSCIENCES (CAT#-STEM-C-3389-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 900mm Length, 7bar, GB-10-900-1TE, G-BIOSCIENCES (CAT#-STEM-C-3390-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 1000mm Length, 7bar, GB-10-1000-1TE, G-BIOSCIENCES (CAT#-STEM-C-3391-LGZ)