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The eggshell has been shaped through evolution to resist physical and pathogen challenges from the external environment, while satisfying the metabolic and nutritional needs of the developing embryo by regulating gas and water exchange, and serving as a calcium store. Eggshell ultrastructure varies considerably. The shell of the egg is composed of calcium carbonate (about 94%) with small amounts of magnesium carbonate, calcium phosphate and other organic matter, including protein.