Mechanism Study of Peptide-induced Mast Cell Degranulation by Patch clamp (CAT#: STEM-PET-0048-WXH)

Introduction

Mast cells have the capacity to release a multitude of pro-inflammatory mediators. The immediate response upon mast cell activation to an appropriate stimulus is degranulation; a process characterized by the extrusion of cytoplasmic granule contents into the extracellular space by the process of exocytosis.

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Principle Applications Procedure Materials Notes Related Products

Principle

The patch-clamp technique involves a glass micropipette forming a tight gigaohm seal with the cell membrane. The micropipette contains a wire bathed in an electrolytic solution to conduct ions. To measure single ion channels, a “patch” of membrane is pulled away from the cell after forming a gigaohm seal.

Applications

• Study of ionic currents in individual isolated living cells, tissue sections, or patches of cell membrane.
• Study of excitable cells such as neurons, cardiomyocytes, muscle fibers, and pancreatic beta cells.
• Study of ion channels.

Procedure

1. Fabrication of glass electrodes
2. Measuring glass electrode resistance and compensating offset potential
3. Glass electrode contact to cell membrane and obtain a GΩ seal
4. Acquire and analyse recordings using the appropriate software.

Materials

Patch clamp system

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