Normal Phase I PhyTip® Columns (500 µL Volume) Containing 20 µL Silica Per Column, PTT-95-20-09, Tecan (CAT#: STEM-C-3982-LGZ)

Oligosaccharide Enrichment Mapping and Identification Using Normal Phase PhyTip Columns

Sample processing through a normal-phase PhyTip column for dual-flow chromatography results in specific enrichment of polar compounds such as oligosaccharides. Because of this, normal-phase PhyTip columns are the only commercially available solution for high-throughput oligosaccharide purification for oligosaccharide localization and identification analysis of glycoproteins.
Fluorescent dye labeling is commonly used to improve the detection of oligosaccharides in high performance liquid chromatography and capillary electrophoresis. Normal phase PhyTip columns automatically remove sample dyes prior to identification and quantitation for high throughput and reproducibility.

Pack Size: 96

PhyNexus, now part of Biotage, developed PhyTip® columns in 2002 and has supported researchers in automated protein purification since then. PhyTip columns purify samples through repeated back-and-forth cycles, a process called dual-flow chromatography that allows binding interactions to be completed at each purification step for superior protein purification results.

High-throughput automatic oligosaccharide purification technology:
Our PhyTip columns automate processes that are difficult to automate. Normal phase PhyTip columns are available for high-throughput automated purification of oligosaccharides.

Sample enrichment and purification in one:
Our unique column design with packed resin bed results in binding the entire sample into a small resin bed volume. Purified samples can be eluted in small volumes, resulting in highly concentrated samples.

Authentication protocol:
Our normal-phase PhyTip columns employ dual-flow chromatography, are validated with real protein samples, and can be used in any automated liquid handling system.

Speed:
Normal phase PhyTip columns can be used to purify 96 samples in just 15 minutes.

Compatible with all major liquid handling platforms:
Our PhyTip columns are compatible with all major liquid handling platforms, enabling you to use your existing systems, which you may already have in your laboratory for automated protein purification.

Normal Phase I PhyTip® Columns (200 µL Volume) Containing 5 µL Silica Per Column, PTT-92-05-09, Tecan (CAT#-STEM-C-3976-LGZ)
Normal Phase I PhyTip® Columns (300 µL Volume) Containing 20 µL Silica Per Column, PTX-93-20-09, DD/Biotix (CAT#-STEM-C-3977-LGZ)
Normal Phase I PhyTip® Columns (300 µL Volume) Containing 20 µL Silica Per Column, PTH-93-20-09, Hamilton (CAT#-STEM-C-3978-LGZ)
Normal Phase I PhyTip® Columns (300 µL Volume) Containing 5 µL Silica Per Column, PTX-93-05-09, DD/Biotix (CAT#-STEM-C-3979-LGZ)
Normal Phase I PhyTip® Columns (300 µL Volume) Containing 5 µL Silica Per Column, PTH-93-05-09, Hamilton (CAT#-STEM-C-3980-LGZ)
Normal Phase I PhyTip® Columns (500 µL Volume) Containing 160 µL Silica Per Column, PTT-95-16-09, Tecan (CAT#-STEM-C-3981-LGZ)