Photoaging Animal Model (CAT#: STEM-AE-0039-WXH)

Introduction

The skin is the most superficial and largest organ of the human body and plays an important role in protecting the body from environmental damage and in the exchange of substances. Skin aging is a complex process caused by genes and external environmental factors. Skin aging is divided into endogenous aging and exogenous aging. Intrinsic aging refers to the natural aging that occurs over time that is determined by genes. Exogenous aging refers to the accumulation of skin damage caused by environmental factors such as ultraviolet radiation, smoking, and toxic chemicals, among which ultraviolet radiation is the most important factor leading to exogenous aging. Therefore, exogenous aging is also called skin photoaging. The establishment of an effective skin photoaging animal model is the basis for research and prevention of human skin photoaging.

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Principle Applications Procedure Materials Notes Related Products

Applications

• Research and prevent and treat the related mechanisms of human skin photoaging.
• Photoaging-related drug development, drug screening and efficacy research.

Procedure

1. UV tubes are inserted side by side and placed in the irradiation box as a source of ultraviolet radiation, using a UV illuminance meter to measure the radiation intensity.
2. Before modeling, after the UV lamp light source is stable, put the experimental mice into the homemade mouse cage, adjust the irradiation time according to the minimum erythema dose (MED) obtained in the pre-test and the intensity of the light source, and use the exhaust fan to lower the box during the irradiation period. temperature.
3. In the first week, the dose of 1 MED was irradiated, 3 times a week, and the weekly irradiation dose was increased by 1 MED compared with the previous week, until 4 MEDs in the 4th week, and then kept 4 MEDs until the 4th week. The 8-week experiment is over.
4. The back skin of the mice in the model group showed obvious thickening, thick and deep wrinkles, sagging and other photoaging skin signs, indicating that the mouse skin photoaging modeling was successful.

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