ProPlus PhyTip® Columns (1 mL Volume) Containing 320 µL Affinity Media Per Column, PTR-91-32-07, MEA 2. Incl. Buffer Kit. (CAT#: STEM-C-4115-LGZ)

Our ProPlus and ProPlus LX PhyTip columns are used for efficient automated purification of IgG antibodies. ProPlus (MabSelect Sure™, Cytiva) and ProPlus LX (MabSelect Sure™LX, Cytiva) are affinity media engineered versions of Protein A affinity ligands that exhibit both Protein A and Protein G properties. MabSelect™Sure™ medium facilitates targeted binding of IgGs, high-capacity binding of IgGs from low-concentration samples, and increased selectivity for mouse IgGs. In some cases, the recovery rate of MabSelect™SuRe™ was 3-5 times higher than that of Protein G.
MabSelect™SuRe™ is a licensed trademark of Cytiva.

Pack Size: 96

PhyNexus, now part of Biotage, developed PhyTip® columns in 2002 and has supported researchers in automated protein purification since then. PhyTip columns purify samples through repeated back and forth cycles, a process known as dual-flow chromatography that allows for complete binding interactions during each purification step.

IgGs recovery 70 - 90%
Less protein loss during purification due to maximized sample binding. The result is very high sample recovery.

Sample concentration and purification in one
Our unique packed resin bed column design results in the entire sample being bound to a small resin bed volume. Purified samples can be eluted in small volumes, resulting in highly concentrated samples.

IgG purification for 15 minutes
Efficient purification via PhyTip columns allows for a complete purification protocol of 96 samples in as little as 15 minutes.

Validated Protocols
Our Protein A PhyTip columns employ dual-flow chromatography, are validated with real protein samples, and are transferable to any automated liquid handling system.

ProPlus PhyTip® Columns (1 mL Volume) Containing 160 µL Affinity Media Per Column, PTT-91-16-07, Tecan. Incl. Buffer Kit. (CAT#-STEM-C-4109-LGZ)
ProPlus PhyTip® Columns (1 mL Volume) Containing 20 µL Affinity Media Per Column, PTB-91-20-07, Beckman. Incl. Buffer Kit. (CAT#-STEM-C-4110-LGZ)
ProPlus PhyTip® Columns (1 mL Volume) Containing 20 µL Affinity Media Per Column, PTX-91-20-07, DD/Biotix. Incl. Buffer Kit. (CAT#-STEM-C-4111-LGZ)
ProPlus PhyTip® Columns (1 mL Volume) Containing 20 µL Affinity Media Per Column, PTH-91-20-07, Hamilton. Incl. Buffer Kit. (CAT#-STEM-C-4112-LGZ)
ProPlus PhyTip® Columns (1 mL Volume) Containing 20 µL Affinity Media Per Column, PTR-91-20-07, MEA 2 (CAT#-STEM-C-4113-LGZ)
ProPlus PhyTip® Columns (1 mL Volume) Containing 20 µL Affinity Media Per Column, PTT-91-20-07, Tecan. Incl. Buffer Kit. (CAT#-STEM-C-4114-LGZ)