Protein A Resin, 5 Column Kit, 786-826, G-BIOSCIENCES (CAT#: STEM-C-3272-LGZ)

Immobilized protein A is used to bind the constant domains of immunoglobulin (Ig) molecules. Protein A is coupled to agarose beads by reductive amination method, resulting in high immunoglobulin coupling efficiency and minimal protein A leaching (<5ng protein A/ml). Our immobilized protein A has a high binding capacity (40mg/ml resin).

Immobilized Protein A 0.2ml spin column kit is suitable for small-scale affinity purification of antibodies from various samples. Each 0.2ml chromatographic column is designed for 25-500 μl of serum or other samples, and can purify 6-8mg of IgG according to the resin binding capacity of 30-40mg/ml.

The Immobilized Protein A 1ml Spin Column Kit is ideal for small-scale affinity purification of antibodies formed from a variety of samples. Each 0.2ml chromatographic column is designed for 0.5-2ml of serum or other samples, and can purify up to 40mg of IgG according to the binding capacity of the resin at 30-40mg/ml.

Process up to 96 samples in a 96-well spin plate format. Other formats include FPLC columns and magnetic beads.

Specific binding and elution buffers can also be used.

Size: 5 column kit
High binding capacity: >40mg human IgG/ml resin
Protein A Leaching: <10ng/mg IgG
30-300cm/hr flow velocity
Ligand: Recombinant Staphylococcal Protein A lacking the albumin-binding domain produced in E. coli
Bead size: 45-165μm
Bead Structure: 6% highly cross-linked agarose

Immobilized Protein G Resin, 5ml Resin, 786-284, G-BIOSCIENCES (CAT#-STEM-C-3245-LGZ)
Immobilized Protein G Resin, 10ml Resin, 786-830, G-BIOSCIENCES (CAT#-STEM-C-3246-LGZ)
Immobilized Protein G Resin, 25ml Resin, 786-831, G-BIOSCIENCES (CAT#-STEM-C-3247-LGZ)
Immobilized Protein G Resin, 5 x 1ml Columns, 786-832, G-BIOSCIENCES (CAT#-STEM-C-3248-LGZ)
Immobilized Protein G Resin, 5 Column Kit, 786-833, G-BIOSCIENCES (CAT#-STEM-C-3249-LGZ)
Immobilized Protein G Resin, 10 x 0.2ml Columns, 786-834, G-BIOSCIENCES (CAT#-STEM-C-3250-LGZ)