PROTEINDEX™ Alkali-Tolerant Protein A Agarose 4 Fast Flow, 500 mL, 11-0211-500, Marvelgent biosciences (CAT#: STEM-C-3694-LGZ)

PROTEINDEX™ rProtein A Sepharose 4 Fast Flow is a proprietary affinity chromatography medium designed for simple one-step purification of immunoglobulins, especially monoclonal antibodies, from larger sample volumes.

Alkali-resistant protein A is modified by gene recombination technology on the basis of natural protein A. Our Alkali-resistant Protein A Sepharose 4FF has a capacity of ≥95% after 200 cycles of clean-in-place (CIP) with 0.1M NaOH place, CIP) after 100 cycles, the capacity is still about 80% of the initial capacity. Alkali resistant protein A is immobilized on cross-linked 4% agarose resin with an orientation optimal for immunoglobulin binding. The level of ligand leakage during elution is very low.

Size: 500 mL
Ligand: Recombinant alkali-tolerant protein A produced in E.coli
Binding capacity: >40 mg human IgG/mL medium
Matrix Cross-linked: 4% agarose supplied as a 50% slurry
Particle size: 45 μm - 165 μm, with an average of ~ 90 μm
Maximum pressure: 0.3 MPa (3 bar)
Recommended flow rate: Gravity flow and medium pressure, 30 - 300 cm/hour
Chemical stability: All commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.
Cleaning-in-place (CIP): 0.1 M ~ 0.5 M NaOH
Storage temperature: 2 - 8°C
Storage buffer: 20% ethanol

Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 800mm Length, 7bar, GB-8-800-1TE, G-BIOSCIENCES (CAT#-STEM-C-3379-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 900mm Length, 7bar, GB-8-900-1TE, G-BIOSCIENCES (CAT#-STEM-C-3380-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 8mm ID, 1000mm Length, 7bar, GB-8-1000-1TE, G-BIOSCIENCES (CAT#-STEM-C-3381-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 100mm Length, 7bar, GB-10-100-1TE, G-BIOSCIENCES (CAT#-STEM-C-3382-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 200mm Length, 7bar, GB-10-200-1TE, G-BIOSCIENCES (CAT#-STEM-C-3383-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 300mm Length, 7bar, GB-10-300-1TE, G-BIOSCIENCES (CAT#-STEM-C-3384-LGZ)