PROTEINDEX™ HiBond™ Ni-NTA Agarose, 10 Ml, 11-0224-010, Marvelgent biosciences (CAT#: STEM-C-3652-LGZ)

PROTEINDEX™ HiBond™ Ni-NTA agarose uses 4% agarose as a matrix carrier and is functionalized with a chelating ligand NTA (nittilotriacetic Acid). NTA is charged with nickel ions (Ni2+) to form a nickel ion-centered Stable octahedral structure. The structure of the Ni-NTA complex is a compatible solution containing a certain concentration of reducing agent, denaturant, detergent and other additives.

PROTEINDEX™ HiBond™ Ni-NTA Agarose can be used to purify 6xHis-tagged proteins expressed in various hosts, such as E.coli. , yeast, insect cells and mammalian cells.

Size: 10 mL
Ligand: Nickel
Binding capacity: >40 mg 6xHis-tagged protein/mL medium
Matrix: 4% agarose supplied as 50% slurry
Particle size: 45 μm - 165 μm
Maximum pressure: 0.1 MPa (1 bar)
Recommended flow rate: Gravity flow, <75 cm/hour
Storage temperature: 2 - 8°C
Storage buffer: 1x PBS containing 20% ethanol

For routine purification of histidine-tagged proteins
High yield (typically > 40 mg protein /mL resins)
Low leakage of nickel ions. Can be reused for up to 8 preps*
*Note: When using the recommended buffer, there was no significant drop in yield after 8 reuses of the resin. See product details for compatible reagents.

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