PROTEINDEX™ rProtein G Agarose, 5 mL, 11-0205-005, Marvelgent biosciences (CAT#: STEM-C-3705-LGZ)

PROTEINDEX™ rProtein G Agarose is an affinity chromatography medium designed for simple one-step purification of immunoglobulins, especially most mammalian IgG isotypes, and fusion proteins containing the Fc region.

Protein G was derived from Streptococcus strain G148. It contains the binding site for the Fc portion of mammalian IgG. Native protein G has 3 IgG binding domains as well as albumin and cell surface binding sites. Our recombinant protein G contains only the IgG binding domain, but no albumin recognition domain, and is therefore compatible with purification protocols that require the presence of fetal bovine serum (FBS) or serum albumin.

PROTEINDEX™ rProtein G Agarose exhibits superior binding specificity for IgG species, allows one-step purification of monoclonal and polyclonal antibodies with a purity of over 95%, and is ideal for purification of IgG from various sources, including crude samples of tissue culture and serum .

Size: 5 mL
Ligand: Recombinant protein G produced in E.coli
Binding capacity: >30 mg human IgG/mL medium
Matrix: 4% agarose supplied as 50% slurry
Particle size: 45 μm - 165 μm
Maximum pressure: 0.1 MPa (1 bar)
Chemical stability: All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.
Recommended flow rate: Gravity flow, 100 - 300 cm/hour
pH stability: Long term: pH3 - 9; Short term: pH2 - 10
Storage temperature: 2 - 8°C
Storage buffer: 1x PBS containing 20% ethanol

High capacity, > 30 mg human IgG / mL of settled resins
High selectivity for high purity
Minimal ligand leakage, can be reused

Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 900mm Length, 7bar, GB-10-900-1TE, G-BIOSCIENCES (CAT#-STEM-C-3390-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 10mm ID, 1000mm Length, 7bar, GB-10-1000-1TE, G-BIOSCIENCES (CAT#-STEM-C-3391-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 15mm ID, 100mm Length, 7bar, GB-15-100-1TE, G-BIOSCIENCES (CAT#-STEM-C-3392-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 15mm ID, 200mm Length, 7bar, GB-15-200-1TE, G-BIOSCIENCES (CAT#-STEM-C-3393-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 15mm ID, 300mm Length, 7bar, GB-15-300-1TE, G-BIOSCIENCES (CAT#-STEM-C-3394-LGZ)
Chromatography Columns, 1 Adjustable End, 5-7bar, 15mm ID, 400mm Length, 7bar, GB-15-400-1TE, G-BIOSCIENCES (CAT#-STEM-C-3395-LGZ)