The Recombinant Human Galectin-9 Protein manufactured by Seafrom is suitable for immune regulation and modulation. Galectin-9 (LGALS9), also known as tumor antigen HOM-HD-21 and ecalectin, is a member of the galectin protein family, characterized by their binding specificity for β-galactoside sugars such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc). These sugars can be bound to proteins through either N-linked or O-linked glycosylation. Galectin-9, in particular, exhibits high affinity for the Forssman pentasaccharide and serves as a ligand for HAVCR2/TIM3. Furthermore, Galectin-9 plays a role in stimulating bactericidal activity in infected macrophages by inducing macrophage activation and IL1B secretion, which in turn restricts intracellular bacterial growth. Storage Information: Lyophilized samples can be stored at 4°C for 24 months, and once reconstituted, the liquid can be stored at -20°C for 6-12 months, with care taken to avoid repeated freeze-thaw cycles.
Specification
Expression Host: Human HEK293 cells Source: Human Synonyms: LGALS9, Ecalectin Protein Sequence: The DNA sequence encodes human Galectin-9 (NCBI Reference Sequence: NP_002299.2), expressed with a His tag at the C-terminus. Molecular Weight: Recombinant human Galectin-9 protein contains 323 amino acids, with a predicted theoretical molecular weight of 37.4 kD. Purity: ≥95%, confirmed by SDS-PAGE gel and high-performance liquid chromatography (HPLC) analysis. Endotoxin: <0.1 EU/μg Biological Activity: The ability to induce adhesion migration of Jurkat cells (human acute T lymphoblastic leukemia cells) is confirmed, with an ED50 of 1.1-4.2 μg/mL. Purification Method: Chromatographic purification Composition: Sterile PBS (pH 7.4) freeze-dried powder containing 6% mannitol.
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