Repetitive sequence-based PCR (Rep-PCR) (CAT#: STEM-MB-0216-WXH)

Introduction

Repetitive-element PCR (Rep-PCR) is a method for fingerprinting microbial genomes by PCR amplification of repetitive DNA elements that are widely distributed in the microbial genomes. Rep-PCR technique was rapidly developed and has been widely used in the classification of a variety of microbial genes. High-resolution and easy to perform, this technique can be applied to large or small numbers of samples.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

rep-PCR differentiates microbes by using primers complementary to interspersed repetitive consensus sequences that enable amplification of diverse-sized DNA fragments consisting of sequences between the repetitive elements

Applications

• Genomic fingerprinting.
• Molecular strain typing of different bacteria.
• Epidemiologic discrimination of various pathogens.

Procedure

1.DNA extraction
2.Primer screening and optimization
3.PCR amplification with selective REP or ERIC primers
4.Gel electrophoresis
5.Inrepratation(microbial identification)/Exploration of strain-specific patterns

Other recommended products

Analysis of PPM1J Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1158-LGZ)
Analysis of CDKN2A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1924-LGZ)
Analysis of OR56A1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2356-LGZ)
Analysis of ATAD3C Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1263-LGZ)
Analysis of UBE2T Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0741-LGZ)
Analysis of LOC110121201 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1590-LGZ)
Analysis of SCN3A Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1483-LGZ)
Analysis of RBM15 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1214-LGZ)