Repetitive sequence-based PCR (Rep-PCR) (CAT#: STEM-MB-0216-WXH)

Introduction

Repetitive-element PCR (Rep-PCR) is a method for fingerprinting microbial genomes by PCR amplification of repetitive DNA elements that are widely distributed in the microbial genomes. Rep-PCR technique was rapidly developed and has been widely used in the classification of a variety of microbial genes. High-resolution and easy to perform, this technique can be applied to large or small numbers of samples.

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Principle Applications Procedure Materials Notes Related Products

Principle

rep-PCR differentiates microbes by using primers complementary to interspersed repetitive consensus sequences that enable amplification of diverse-sized DNA fragments consisting of sequences between the repetitive elements

Applications

• Genomic fingerprinting.
• Molecular strain typing of different bacteria.
• Epidemiologic discrimination of various pathogens.

Procedure

1.DNA extraction
2.Primer screening and optimization
3.PCR amplification with selective REP or ERIC primers
4.Gel electrophoresis
5.Inrepratation(microbial identification)/Exploration of strain-specific patterns

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