Sephacryl S-400 HR, 750 mL, Cytiva (CAT#: STEM-C-1627-LC)

Sephacryl S-400 High Resolution manufactured by Cytiva is a highly versatile gel filtration resin which is ideal for separation of polysaccharides, macromolecules with extended structures and even small particles such as plasmids. (fractionation range globular proteins 2 × 10⁴ - 8 × 10⁶) by size exclusion chromatography. The matrix of Sephacryl High Resolution resins is a cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The combination of narrow particle size distribution and steep selectivity curves offers good preparative characteristics and high resolution. The hydrophilic nature of the resin reduces nonspecific adsorption and increases recovery.

BioProcess resin: Yes
Particle size, d50V1: ~50 µm
Matrix: Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.
Particle Size: 25 µm-75 µm
pH stability, operational2: 3-11
pH stability, CIP3: 2-13
Pressure/flow characteristics4: Pressure/flow characteristics: ≥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20°C using buffers with a viscosity similar to water).
Fractionation range (Mr) (globular proteins): 2 × 10⁴-8 × 10⁶
Fractionation (Mp) Dextrans: 1 x 10⁴-2 x 10⁶
Fractionation Range (Mr) DNA Exclusion Limit: 271 bp
Exclusion limit (Mr) (globular proteins): ~9 × 10⁶ (9000 kDa)
Storage: 4 to 30°C, 20% Ethanol
Chemical stability: Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl
Certificate of Analysis:Yes
Pack size: 750 mL

High resolution
Fast and easy operation
Long-term physical and chemical stability

Sephadex G-25 Superfine, 500 g, Cytiva (CAT#: STEM-C-1596-LC)