Simultaneous Estimation of Paracetamol, Diclofenac Potassium and Famotidine in its Combined Pharmaceutical Dosage Form by Reversed-Phase Chromatography (RP-HPLC/RP-UPLC) (CAT#: STEM-CT-2488-CJ)

Introduction

A combination of Paracetamol (PCM), Diclofenac potassium (DCP) and Famotidine (FAMO) is used for the treatment of joint and muscular pain. PCM [N-(4-hydroxyphenyl) acetamide] also known as Acetaminophen, is commonly used for its analgesic and antipyretic effects. PCM is act primarily in the CNS, increasing the pain threshold by inhibiting isoforms of cyclooxygenase, COX-1, COX-2, and COX-3 enzymes involved in prostaglandin (PG) synthesis. DCP is a phenylacetic acid derivative that is a potent inhibitor of COX and that has analgesic, anti-inflammatory and antipyretic effects. Chemically it is 2-{2-[(2,6-dichlorophenyl) amino]phenyl} acetic acid and used in the acute and chronic treatment of osteoarthritis and rheumatoid arthritis. FAMO is chemically 3-[({2 [(diaminomethylidene) amino]-1,3-thiazol-4-yl} methyl) sulfanyl]-N'-sulfamoylpropanimi-damide. Blocking histamine affects by competitively binding to H2-receptors located on the basolateral membrane of the parietal cell, results in reduced basal and nocturnal gastric acid secretion. It is used for the treatment of peptic ulcer disease (PUD) and gastroesophageal reflux disease (GERD) and hypersecretoryconditions, such as Zollinger-Ellison syndrome.