Single-particle tracking of membrane proteins in living plant tissues by photoactivated localization microscopy (CAT#: STEM-MIT-0391-LJX)

Introduction

Arabidopsis thaliana is a delicate annual herb of the genus Arabidopsis in the cruciferous family. Arabidopsis thaliana is a typical self - crossbreeding plant and has a leading position in plant genetic research. Arabidopsis thaliana grows in a wide geographical range and has various species, which is very conducive to the study of plant adaptation to the environment.
Super-resolution microscopy techniques have pushed the limit of optical imaging to unprecedented spatial resolutions. However, one of the frontiers in nanoscopy is its application to intact living organisms. The service is provided for the implementation and application of super-resolution single-particle tracking photoactivated localization microscopy (sptPALM) to probe single-molecule dynamics of membrane proteins in live roots of the model plant Arabidopsis thaliana.

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Principle Applications Procedure Materials Notes Related Products

Principle

Principles of photoactivated localization microscopy: By fitting the two-dimensional Gaussian function to determine the centroid of microscope-formed light spots, a single fluorescent source (such as a fluorescent group) can be located with high precision. The accuracy of the calculation to determine the centroid depends only on the number of photons collected, and the resolution scale can be tens of nanometers or smaller. To achieve this accuracy, the density of the fluorescent molecules being tested is required to be low enough that the spots of the two fluorescent groups are unlikely to overlap.

Applications

Applied in many areas of the life sciences

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Roots of arabidopsis thaliana

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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