Study of fibrinogen adsorption on poly(ethylene glycol)-modified surfaces by Dual polarization interferometry (DPI) (CAT#: STEM-MB-0459-WXH)

Introduction

Plasma protein adsorption from blood plasma is a key factor that determines whether materials can be used as medical polymer materials. Fibrinogen is an adhesive protein with a rodlike structure and plays a vital role in coagulation, platelet activation and aggregation, inflammatory response, and leucocyte binding. The Fib molecule consists of two peripheral D domains and one central E domain held together by triple-stranded α-helical coiled coils. Paul et al. found that the positively charged αC domains may play a role in keeping Fib soluble in the blood stream, which could inhibit blood coagulation. Therefore, understanding the Fib adsorption behavior and mechanism at polymer surface is significant for fabricating high performance blood-contacting materials.

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Principle Applications Procedure Materials Notes Related Products

Principle

Dual polarization interferometry (DPI) is an analytical technique that allows the simultaneous determination of thickness, density, and mass of a biological layer on a sensing waveguide surface in real time. DPI focuses laser light into two waveguides. One of these functions as the "sensing" waveguide having an exposed surface while the second one functions to maintain a reference beam. A two-dimensional interference pattern is formed in the far field by combining the light passing through the two waveguides. The DPI technique rotates the polarization of the laser, to alternately excite two polarization modes of the waveguides. Measurement of the interferogram for both polarizations allows both the refractive index and the thickness of the adsorbed layer to be calculated. These measurements can be used to infer conformational information about the molecular interactions taking place, as the molecule size (from the layer thickness) and the fold density (from the RI) change.

Applications

Study of fibrinogen adsorption on poly(ethylene glycol)-modified surfaces.

Procedure

1. Setting of dual polarization interferometry
2. Preparing the DPI sensor chip
3. Immobilization of target on DPI biosensor
4. Reagent was injected to react
5. Quantitative analysis

Materials

• DPI biosensor
• DPI sensor chip

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