Study of lipids by Raman Spectroscopy (CAT#: STEM-ST-0079-WXH)

Introduction

Lipids are undoubtedly one of the most important classes of biomolecules involved in cellular signaling, energy storage and building of cellular membranes. Raman scattering cross-section of several lipids’ signals is large due to the presence of long non-polar acyl chains in their structures. Thus, bands assigned to lipids are omnipresent in the Raman spectra of cells and tissues, and in many cases, they are convenient markers of pathology.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope

Other recommended products

Study of cornea by Raman Spectroscopy (CAT#: STEM-ST-0065-WXH)
Study of unsaturated fatty acids by Raman Spectroscopy (CAT#: STEM-ST-0080-WXH)
Detection and Identification of microorganisms by Raman Spectroscopy (CAT#: STEM-ST-0093-WXH)
Study of saturated fatty acids by Raman Spectroscopy (CAT#: STEM-ST-0081-WXH)
Analysis of Trypsin inhibitor (TIP) by Raman Spectroscopy (CAT#: STEM-ST-0020-WXH)
Analysis of biocompatibility of a material by Raman Spectroscopy (CAT#: STEM-ST-0001-WXH)
Study of atherosclerotic plaque by Raman Spectroscopy (CAT#: STEM-ST-0066-WXH)
Characterisation of stratum corneum by Raman spectroscopy (CAT#: STEM-ST-0040-WXH)