Study of Mobility of Adsorbed Proteins by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0048-WXH)

Introduction

Extensive research has been performed on the macroscopic scale to probe protein adsorption, particularly in the areas of adsorption kinetics, equilibrium, and relevant transport phenomena for different systems. However, information considering the molecular-level behavior of adsorbed proteins forms a relatively small fraction of this work, and understanding of such issues as lateral mobility, conformation, orientation, and ordering of protein molecules is thus incomplete. It is especially translational mobility of adsorbed protein that we investigate here, which is relevant to such applications as protein transport in chromatography and formation of structured adlayers at surfaces.