Study of Self-Diffusion in Chitosan Networks by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0057-WXH)

Introduction

Chitosan is a natural linear cationic polysaccharide obtained by N-deacetylation of chitin. The numerous physicochemical and biological properties of chitosan such as biocompatibility, non toxicity, mucoadhesion, absorption enhancement and pHdependent swelling properties are particularly interesting for many applications in the biomedical and the pharmaceutical fields. This is why chitosan networks are already used as hydrophilic matrix formers in controlled drug delivery systems.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Fluorescence recovery after photobleaching (FRAP) is a microscopy technique capable of quantifying the mobility of molecules within cells. By exploiting the phenomenon of photobleaching, fluorescent mole- cules within a region of interest can be selectively and irreversibly 'turned off'. It is capable of quantifying the two-dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells.

Applications

• Characterization of the mobility of individual lipid molecules within a cell membrane.
• Analysis of molecule diffusion within the cell
• Study of protein interaction partners, organelle continuity and protein trafficking.

Procedure

1. An initial fluorescence of fluorescent molecules is measured in the region of interest (ROI).
2. The fluorescent molecules are rapidly photobleached by focusing the high-intensity laser beam onto the defined area.
3. The exchange of bleached molecules with unbleached molecules from the surrounding region is followed over time using a low-intensity laser.

Materials

• Optical microscope.
• Light source.
• Fluorescent probe.

Other recommended products

Measurement of rotational motion in membranes by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0037-WXH)
Study of Trafficking of the Epithelial Na+ Channel toward the Plasma Membrane by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0043-WXH)
Study of Molecular diffusion within sol–gel derived matrices by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0061-WXH)
Study of the Solution Properties of Hyaluronan by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0019-WXH)
Organization and Dynamics of NBD-Labeled Lipids in Membranes by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0024-WXH)
Study of intestinal mucus diffusion of polyphenols by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0058-WXH)
Characterization of diffusion of macromolecules in konjac glucomannan solutions and gels by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0026-WXH)
Analysis of dynamics of plasma membrane by Fluorescence recovery after photobleaching (FRAP) (CAT#: STEM-MT-0020-WXH)