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Study of the fast kinetic decolourisation of textile dyes by ozone using Quench-Flow method (CAT#: STEM-AC-0040-WXH)

Introduction

The environmental concern resulting from the increasing contamination of natural water supplies by refractory pollutants makes the use of less conventional depuration technologies highly pertinent, as it is the case of chemical oxidation. Thus, the powerful oxidizing capabilities of ozone alone or in combination with UV radiation and hydrogen peroxide are being increasingly applied to the treatment of wastewater effluents containing hazardous organic and inorganic compounds, namely pharmaceuticals, pesticides, aromatic compounds, absorbable organic halogens, textile dyes and many other chemicals, which are difficult or expensive to remove through biodegradation or through other physicochemical processes. Ozonation can be applied to achieve pollutants complete oxidation (i.e. mineralization) or for biodegradability enhancement through chemical breakdown of refractory compounds into smaller and less recalcitrant molecules by partial oxidation.<br />Under acidic conditions, ozone can directly react with organic substrates as an electrophile. Ozone is frequently used for decolorizing dye wastewaters because it attacks conjugated double bonds which are often associated with color.




Principle

The principle is to mix the two solutions and then to observe a change in spectroscopic properties of the mixture at different place along the reaction tube during the flow. In the continuous mode of the quenched-flow method, the observation chamber is replaced by a second mixer in which the quenching agent arrives.

Applications

Used to determine fast reaction rates or single turnover rates of enzymatic reactions and to isolate reaction intermediates.

Procedure

Small volumes of solutions are driven through a high efficiency mixer and flow into a delay (or ageing) loop. After a set time, the reaction is stopped (or quenched) by the addition of a chemical quench solution.

Materials

Quench flow