Study of the human cornea following excimer laser keratectomy by atomic force microscope technology (CAT#: STEM-SMMT-0002-LJX)

Introduction

The cornea is the transparent part of the front outer wall of the eye. It is rounded, occupies one-sixth of the outer surface, is about 1 mm thick, and consists mainly of vaseless connective tissue. The front of the cornea is slightly raised, curved like a sphere, with refraction. The cornea is rich in sensory nerve endings, and any small irritation, injury or inflammation can cause pain and tears.
Fresh human corneas were irradiated in vitro with an increasing number of impulses emitted by a 193 nm ArF laboratory excimer laser in order to produce either smooth flat surfaces or stair-like formations within the cornea. The corneas were investigated in a Topometrix(R) atomic force microscope in their native state. For comparison, three corneas were fixed with glutaraldehyde and processed for scanning electron microscopy.

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Principle Applications Procedure Materials Notes Related Products

Principle

Atomic force microscope (AFM) is a new type of surface analysis instrument based on the principles of physics and imaging through the interaction of scanning probe and sample surface atoms. It belongs to the third generation of microscopes after optical microscopes and electron microscopes.
AFM usually uses a sharp probe to scan the sample, which is fixed on a microcantilever that is extremely sensitive to the surface force between the probe and the sample. The deflection of the cantilever under force can cause the laser beam emitted by the laser source to shift after being reflected by the cantilever. The detector receives reflected light, and finally receives signals that are collected, processed, and formed into surface morphology images of the sample through a computer system.

Applications

Imaging of micro and nanoscale features on the surface of the sample
Suitable for research in fields such as materials science, biology, and chemistry

Procedure

1. Sampling
2. Preparation of slices
3. Staining (Select according to the specific experimental situation)
4. Observation

Materials

• Sample Type:
Human cornea

Notes

Operate in strict accordance with the operating procedures, and shall not arbitrarily change the operating procedures

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