Super-resolution Imaging of the T cell Central Supramolecular Signaling Cluster by Stimulated Emission Depletion Microscopy (CAT#: STEM-MIT-0359-LJX)

Introduction

Supramolecular signaling assemblies are of interest for their unique signaling properties. A µm scale signaling assembly, the central supramolecular signaling cluster (cSMAC), forms at the center interface of T cells activated by antigen presenting cells (APC). The adaptor protein linker for activation of T cells (LAT) is a key cSMAC component. The cSMAC has widely been studied using total internal reflection fluorescence microscopy of CD4+ T cells activated by planar APC substitutes.