Thermo Fisher Scientific Attune NXT - 2 Laser, Thermo Fisher Scientific (CAT#: STEM-CAPO-0031-ZJF)

The Thermo Fisher Scientific Attune NXT - 2 Laser is manufactured by Thermo Fisher Scientific.
Manufactured in 2018, we have 2 lasers Attune NxT Acoustic Focusing Cytometer- Blue: 488 nM (50 mW) and Red: 637 nM (100 mW)
The complete system includes Auto Sampler, a computer loaded with software. The system is re-certified by OEM trained service engineer.
Attune Flow Cytometers are compact, benchtop cell analyzers that can be configured with up to four spatially separated lasers to flexibly design, run, and analyze panels of up to 14 colors. Unique, powerful acoustic technology avoids clogging and dramatically reduces cell preparation and processing time even for troublesome large cells and dissociated tissue masses. The systems attain sample throughput rates of 1,000 µL/min, ten times faster than traditional cytometers without compromising data integrity. Attune Cytometric Software makes the system easy to use with an optional module for 21 CFR part 11 compliance.
The Attune CytPix model includes a high-speed brightfield camera that helps you visually confirm that your gates contain cells of interest and discover relevant morphology.

Year: 2018
Condition: Refurbished, Certified pre-owned
Abs / Exc: Blue: 488 nm (50 mW), Violet: 405 nm (50 mW), Red: 637 nm (100 mW) Yellow: 561 nm (50 mW)
Detection: FSC, SSC, 14 fluorescent colors with PMTs, User changeable
Voltage: 100 to 240 V AC, 50 to 60 Hz
Volume: Minimum 50 µL for 96-well plate
Applications: Multi-parametric single cell analysis with minimal compensation panels, Rapidly process and detect multiple samples, Rapid detection of rare events
Throughput: 35,000 events per second
Sample Type: 17 x 100 mm to 8.5 x 45 mm tubes
Dimensions: (WxDxH) 58 x 43 x 40 cm
Flow Rate: 12.5 to 1000 µL/min
Software: Attune™ NxT Software
Time:
< 45 minutes for 96-well plate
< 60 minutes for 96-well plate with 2 wash cycles
< 180 minutes for 384-well plate using High Throughput mode
< 240 minutes for 384-well plate using Standard mode, 2 wash cycles, Carryover < 0.5%
Particle Size: 0.5 to 50 µm

Modular Design: 14 configurations to choose from 1-4 lasers across a list of options
Laser profile: Flat top laser requiring minimal alignment
Flow cell: Quartz cuvette gel coupled to 1.2 NA collection lens
Alignment: Fixed alignment, no customer maintenance required
Fluid storage: All fluids stored within instrument with active fluid level sensing
Standard fluidic tanks: 1.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank
External tanks option: Optional configuration for 10 L fluid
Nominal fluid consumption: 1.8 L/day
Sample tubes: Accommodates tubes from 17 x 100 mm to 8.5 x 45 mm
Sample Analysis Volume: 20 µL to 4 mL Fluorescence sensitivity: ≤ 80 MESF FITC, ≤ 30 MESF PE, ≤ 70 MESF APC

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