UniHR Series HIC Chromatography Resins, UniHR Butyl-30S 100mL (CAT#: STEM-C-3363-ZJF)

Hydrophobic interaction chromatography (HIC) leverages differences in the hydrophilicity and hydrophobicity of biomolecules to enable effective separation. Under high-salt conditions, biomolecules are adsorbed onto the hydrophobic groups of the chromatography medium. By gradually reducing the salt concentration, proteins are eluted sequentially based on their hydrophobicity, achieving precise separation and purification. With mild operating conditions and high resolution, HIC is a widely used method for isolating and purifying biological macromolecules.
UniHR HIC resins are constructed on a monodisperse, hydrophilically modified polymethacrylate matrix. These resins feature phenyl and butyl hydrophobic groups bonded to the hydrophilic matrix, forming two distinct families of HIC resins to meet diverse separation and purification needs.

Chromatography Technique: Hydrophobic interaction
Matrix: Polymethacrylate
Particle Size: 35 µm
Ligand: Butyl
Dynamic Binding Capacity: ~23 mg·mL⁻¹ (Lys)
Maximum Pressure: 1.0 MPa
Clean In Place: 1 M NaOH / 70% Ethanol
Recommended Flow Velocity: 50-300 cm/h
pH Stability: 2-12
Chemical Stability: Stable in commonly used buffers, avoid long-term exposure to strong oxidants
Operational Temperature: 15-30 ℃
Storage: 20% Ethanol, 4-25 ℃

Uniformed particle size
High mass transfer and high resolution
Outstanding mechanic strength and superior pressure-flow characteristics
Minimal non-specific binding and high recovery

STEM-C-3361-ZJF
STEM-C-3362-ZJF
STEM-C-3364-ZJF
STEM-C-3365-ZJF