UniHybrid Eterne Reversed Phase Bulk Media 10-120 C4, 500 g (CAT#: STEM-C-3071-ZJF)

Silica reversed phase chromatography (RPC) is a widely used purification method for peptide-based therapeutics, including insulins and g-l-p-1 compounds. High pH conditions are often preferred in these processes, such as during column regeneration with 0.1M NaOH solution. However, conventional silica media typically have a short lifespan under these conditions. While hybrid silica particles are known for their high pH stability, they are generally limited to sizes below 10 µm and are primarily used in analytical HPLC applications. Currently, hybrid silica particles in sizes of 10 µm or larger are not commercially available for process-scale chromatography, likely due to their low surface area, high production costs, and the challenges associated with large-scale manufacturing. Hybrid-coated silica particles offer improved lifespans at high pH compared to conventional silica but still lack the stability of fully hybrid particles.
With advanced Precisely Controlled Silica (PCS) technology, monodispersed silica particles can be produced in large quantities at a low cost, achieving yields of nearly 100% per batch (several hundred kilograms). Leveraging this innovation, monodispersed hybrid silica particles—UniHybrid® Eterne—have been developed in a 10 µm size. These particles offer a high surface area, excellent mechanical strength, and more than ten times the chemical stability of traditional silica particles, addressing the demand for high-performance RPC media with exceptional pH tolerance.

Particle Size: 10 μm
Pore Size: 120 Å
Surface Area: 320 m²/g
Pore Volume: 1.20 ml/g
Stationary Phase: C18/C8/C4
Max. Pressure: 600 bar
pH Range: 2-13
Pacakage Size: 500 g

High pH Stability: UniHybrid® Eterne hybrid silica particles exhibit over 10 times the chemical stability of conventional silica particles, making them ideal for high pH conditions such as column regeneration with 0.1M NaOH solution.
Monodispersed Particles: Developed using Precisely Controlled Silica (PCS) technology, the UniHybrid® Eterne particles are monodispersed, ensuring uniformity and consistency in chromatography applications.
Large-Scale Production: PCS technology enables the production of large quantities (several hundred kilograms per batch) of hybrid silica particles with almost 100% yield, reducing manufacturing costs.
Improved Mechanical Strength: UniHybrid® Eterne particles possess high mechanical strength, ensuring durability and longer lifetimes in process-scale chromatography applications.
10 µm Size for Process-Scale Chromatography: UniHybrid® Eterne particles are available in a 10 µm size, making them suitable for process-scale reversed phase chromatography (RPC) and peptide-based therapeutic purification.
High Surface Area: Despite being larger than 10 µm, UniHybrid® Eterne particles maintain a high surface area, enhancing their efficiency for purification tasks.
Cost-Effective Manufacturing: The large-scale, low-cost production of UniHybrid® Eterne particles makes them an affordable alternative to conventional silica media, offering high performance at a lower cost.

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