UniMab Series Protein A Affinity Resins, UniMab 50HC 30mL (CAT#: STEM-C-3260-ZJF)

NanoGel dT20 is specifically engineered to meet the selectivity and capacity demands of large-scale downstream purification for various mRNA constructs used in vaccine and gene therapy applications. This resin efficiently captures mRNA through its polyadenylated (polyA) tail using straightforward purification steps involving salt for binding and water for elution. NanoGel dT20 ensures effective separation of mRNA from transcription reaction components, including enzymes and plasmid DNA. It provides a robust and efficient platform for the purification of diverse mRNA molecules across a range of applications.

Chromatography Technique: Antibody affinity
Matrix: Polymethacrylate
Particle Size: 50 μm
Ligand: Alkaline-stabilized recombinant protein A
Dynamic Binding Capacity: ~50 mg·mL⁻¹ (Human IgG, 6 min residence time)
Maximum Pressure: 0.8 MPa
Clean In Place: 0.1-0.5 M NaOH
Recommended Flow Velocity: 100-800 cm/h
pH Stability: 2-12
Chemical Stability: Stable in commonly used buffers, 0.1 M HAc, 0.1 M HCl, 100% ethanol, isopropyl alcohol, etc.; avoid long-term exposure to strong acids or bases
Operational Temperature: 2-40 ℃
Storage: 20% Ethanol, 2-8 ℃

Rigid Particles with Excellent Pressure-Flow Properties: Ensures reliable performance and easy linear scale-up.
Efficient Mass Transfer and High Binding Capacity: Provides high dynamic binding capacity with minimal elution volumes.
Minimal Non-Specific Binding: Guarantees excellent antibody recovery.
Animal Component-Free Production: Ensures compliance with regulatory standards and provides full technical support.

STEM-C-3258-ZJF
STEM-C-3259-ZJF
STEM-C-3261-ZJF
STEM-C-3262-ZJF