Analysis Kinetics of Deoxynivalenol (DON) by BLI (CAT#: STEM-MB-0283-CJ)

Introduction

Deoxynivalenol (DON) is a natural-occurring mycotoxin mainly produced by Fusarium graminearum. It is also know as vomitoxin due to his strong emetic effects after consumption, because it is transported into the brain, where it runs dopaminergic receptors. The emetic effects of this mycotoxin were firstly described in Japanese men consuming mouldy barley containing Fusarium fungi in 1972. DON is probably the best known and most common contaminant of grains and their subsequent products. Its occurrence in food and feed represent more than 90% of the total number of samples and it is a potential marker of the occurrence of other mycotoxins. One of the most important physicochemical property of DON is its ability to withstand high temperatures, which is the risk of its occurrence in food. Numerous studies have documented that DON was heat-stable. However, DON levels are reduced in cooked pasta and noodles because of leaching into the cooking water.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Bio-Layer Interferometry (BLI) is an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. BLI experiments are used to determine the kinetics and affinity of molecular interactions. In a BLI experiment, one molecule is immobilized to a Dip and Read Biosensor and binding to a second molecule is measured. A change in the number of molecules bound to the end of the biosensor tip causes a shift in the interference pattern that is measured in real-time.

Applications

Immunology/Inflammation; Toxicology

Procedure

1. Detect Buffers and prepare samples. BLI experiments are set up with one molecule immobilised on the surface of the biosensor (load sample) and a second molecule in solution (the analytical sample).
2. Fix the load sample on the biocompatible biosensor while the analytical sample is in solution.
3. The biosensor tip is immersed in the solution so that the target molecule begins to bind to the analysis sample.
4. Set up and run the BLI experiment. Molecules bound to or dissociated from the biosensor can generate response curves on the BLI system; unbound molecules, changes in the refractive index of the surrounding medium or changes in flow rate do not affect the interferogram pattern.
5. Collect and analyse data on the BLI's system.

Materials

• Equipment:Fortebio Bio-Layer Interferometry (BLI)
• Sample Type: DNA, RNA, Protein, Antibodies, Peptides, Small Molecules

Other recommended products

Analysis Biomolecular Interactions of Cytokine with Cognate Receptor by BLI (CAT#: STEM-MB-0226-CJ)
Analysis Biomolecular Interactions of His-tagged Mouse FcγRIIb and FcγRIII with Anti-penta-His by BLI (CAT#: STEM-MB-0122-CJ)
Analysis Biomolecular Interactions of Human Monoclonal Antibody RSB1 with D25 by BLI (CAT#: STEM-MB-0230-CJ)
Analysis Biomolecular Interactions of Human EAG1 (hEAG1) Proteins with Small Molecule PIP2 by BLI (CAT#: STEM-MB-0091-CJ)
Analysis Biomolecular Interactions of the Inhibitor MMV007389 with PfFNT by BLI (CAT#: STEM-MB-0212-CJ)
Analysis Biomolecular Interactions of A/H10 Seal Influenza Viruses with Avian-type Receptor Analogs by BLI (CAT#: STEM-MB-0164-CJ)
Analysis Kinetics of CTX-MI in Tap Water by BLI (CAT#: STEM-MB-0276-CJ)
Analysis Biomolecular Interactions of mAbs witj HCV J6 E2 Glycoprotein by BLI (CAT#: STEM-MB-0179-CJ)