Analysis Kinetics of FVIII-Specific Monoclonal IgG1 Antibody by BLI (CAT#: STEM-MB-0279-CJ)

Introduction

Neutralizing antibodies against factor VIII (FVIII), also known as FVIII inhibitors, remain the major challenge in treating congenital hemophilia A with FVIII products. They may also arise as autoantibodies, causing the rare bleeding disorder acquired hemophilia A.FVIII-specific IgG4 emerged as a distinguishing subclass because it was found exclusively in patients with FVIII inhibitors. The signals produced by monovalent protein antigens such as FVIII are considered to be insufficient to induce clonal expansion of B cells and their differentiation into antibody-producing plasma cells.

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Principle Applications Procedure Materials Notes Related Products

Principle

Bio-Layer Interferometry (BLI) is an optical technique for measuring macromolecular interactions by analyzing interference patterns of white light reflected from the surface of a biosensor tip. BLI experiments are used to determine the kinetics and affinity of molecular interactions. In a BLI experiment, one molecule is immobilized to a Dip and Read Biosensor and binding to a second molecule is measured. A change in the number of molecules bound to the end of the biosensor tip causes a shift in the interference pattern that is measured in real-time.

Applications

Immunology/Inflammation

Procedure

1. Detect Buffers and prepare samples. BLI experiments are set up with one molecule immobilised on the surface of the biosensor (load sample) and a second molecule in solution (the analytical sample).
2. Fix the load sample on the biocompatible biosensor while the analytical sample is in solution.
3. The biosensor tip is immersed in the solution so that the target molecule begins to bind to the analysis sample.
4. Set up and run the BLI experiment. Molecules bound to or dissociated from the biosensor can generate response curves on the BLI system; unbound molecules, changes in the refractive index of the surrounding medium or changes in flow rate do not affect the interferogram pattern.
5. Collect and analyse data on the BLI's system.

Materials

• Equipment: Fortebio Bio-Layer Interferometry (BLI)
• Sample Type: DNA, RNA, Protein, Antibodies, Peptides, Small Molecules

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