Analysis of 4-1BB/TNFRSF9 (Human) by ELISA (CAT#: STEM-MB-0639-LGZ)

Introduction

TNFRSF9 (also known as ILA; 4-1BB; CD137; CDw137) belongs to the tumor necrosis factor (TNF) receptor superfamily. TNFRSF9 is a type II membrane glycoprotein. TNFRSF9 is an important activated immune checkpoint molecule on the surface of T cells.<br />TNFRSF9 is ubiquitously expressed on mouse and human CD4+ and CD8+ T cells. In addition, TNFRSF9 is also expressed in activated natural killer (NK) and dendritic cells (DC) in mice. Human TNFRSF9 is also expressed in DCs, monocytes, liver cancer cells, and blood vessels of patients with malignant tumors. TNFRSF9 can be rapidly expressed on CD4+ and CD8+ T cells after antigen exposure, and activated TNFRSF9 regulates T cell-mediated immune responses by transmitting activation, proliferation or apoptosis signals between immune cells. As a newly discovered immunostimulatory molecule, TNFRSF9's potential strength should not be underestimated.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Immunology/Inflammation

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Serum, plasma, cell culture supernatant and other biological samples

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