Analysis of a-Chymotrypsinogen A (type II) by Raman Spectroscopy (CAT#: STEM-ST-0010-WXH)

Introduction

Elastase (EST) is an enzyme from the group of hydrolases, specifically–endopeptidases. In its active site contains serine, so it is classified as a serine protease. EST hydrolyzes peptide bonds, which are in the vicinity of small amino acids such as glycine, alanine and serine. EST as well as chymotrypsin is well known as TPO-like proteases, which hydrolyze the proteins, including elastin.

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Principle Applications Procedure Materials Notes Related Products

Principle

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions.
The principle behind Raman spectroscopy is that the monochromatic radiation is passed through the sample such that the radiation may get reflected, absorbed, or scattered. The scattered photons have a different frequency from the incident photon as the vibration and rotational property vary.

Applications

• Analysis of biocompatibility of a material.
• Analysis of nucleic acids.
• Study of interactions between drugs and cells.
• Photodynamic therapy (PDT).
• Analyzing metabolic accumulations of a substance or compounds.
• Diagnosis of disease.
• Analysis of individual cells.
• Cell sorting applications.
• Analyzing the features of biomolecules.
• Study of bone structure.

Procedure

1. Preparation of samples
2. Determine instrument parameters
3. Perform background scan
4. Test the sample
5. Data analysis

Materials

• Raman Spectrometer
• Raman Imaging Microscope

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