Analysis of ACOT7 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1275-LGZ)

Introduction

Official Full Name: acyl-CoA thioesterase 7
Also known as: ACT; ACH1; BACH; LACH; LACH1; hBACH; CTE-II
This gene encodes a member of the acyl coenzyme family. The encoded protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this gene may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of Fusarium Poae by Real-Time PCR Method (CAT#: STEM-MB-3435-LGZ)
Analysis of Staphylococcus Equorum by Real-Time PCR Method (CAT#: STEM-MB-3384-LGZ)
Analysis of MLPH Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1660-LGZ)
Analysis of HumanT-lymphotropicVirus2(HTLV-2) by Real-Time PCR Method (CAT#: STEM-MB-2555-LGZ)
Analysis of ATF6 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0782-LGZ)
Analysis of OR2T2 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0547-LGZ)
Analysis of Radix Saposhnikoviae by Real-Time PCR Method (CAT#: STEM-MB-3981-LGZ)
Analysis of Teladorsagia Circumcincta by Real-Time PCR Method (CAT#: STEM-MB-3775-LGZ)