Analysis of AGRN Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1272-LGZ)

Introduction

Official Full Name: agrin
Also known as: CMS8; AGRIN; CMSPPD
The gene encodes one of several proteins critical in the development of the neuromuscular junction (NMJ), found in mouse knockout studies. The encoded protein contains several laminin G, Cajar-type serine protease inhibitor, and epidermal growth factor domains. Additional post-translational modifications occur by adding glycosaminoglycans and disulfide bonds. A mutation in this gene was found in a family with a congenital myasthenic syndrome affecting the limb-girdle muscles. Alternative splicing results in multiple transcript variants encoding different isoforms.

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Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

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