Analysis of AKIP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-2199-LGZ)

Introduction

Official Full Name: A-kinase interacting protein 1
Also known as: BCA3; C11orf17
This gene encodes a nuclear protein that interacts with protein kinase A catalytic subunit, and regulates the effect of the cAMP-dependent protein kinase signaling pathway on the NF-kappa-B activation cascade. Alternatively spliced transcript variants have been described for this gene.

Add to Cart Please Inquire

Principle Applications Procedure Materials Notes Related Products

Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Gene mutation analysis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements

Other recommended products

Analysis of PRAMEF15 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-1382-LGZ)
Analysis of EPB41 Gene (Mutation) in Elliptocytosis 1 by RT-qPCR (CAT#: STEM-MT-0094-LGZ)
Analysis of CRP Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0874-LGZ)
Analysis of TCHH Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0294-LGZ)
Analysis of AIV-H5N1 Ag by Real-Time PCR Method (CAT#: STEM-MB-2907-LGZ)
Analysis of Prevotella Melaninogenica by Real-Time PCR Method (CAT#: STEM-MB-3782-LGZ)
Analysis of Avian Leukosis Virus (ALV) by Real-Time PCR Method (CAT#: STEM-MB-2847-LGZ)
Analysis of TARBP1 Gene (Mutation) by RT-qPCR (CAT#: STEM-MT-0305-LGZ)