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Analysis of TBX19 Gene (Mutation) in Lymphatic malformation 6 by RT-qPCR (CAT#: STEM-MT-0162-LGZ)

Introduction

Also known as: TPIT; TBS19; dJ747L4.1
The gene is a member of a phylogenetically conserved gene family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Mutations in this gene were found in patients with isolated pituitary POMC-derived ACTH deficiency, suggesting that this gene plays an important role in the differentiation of pituitary POMC lineages. ACTH deficiency is characterized by adrenal insufficiency, such as weight loss, loss of appetite (anorexia), weakness, nausea, vomiting, and low blood pressure.




Principle

Quantitative reverse transcription PCR (RT-qPCR) is an experimental method applied to PCR experiments using RNA as the starting material. In this method, total or messenger RNA (mRNA) is first transcribed into complementary DNA (cDNA) by reverse transcriptase. Subsequently, qPCR reaction was performed using cDNA as template.

Applications

Lymphatic malformation 6

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

Sample: depends on the customer's analysis requirements
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