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Analysis of Bunyamwera Virus by Real-Time PCR Method (CAT#: STEM-MB-2724-LGZ)

Introduction

A real-time polymerase chain reaction (real-time PCR,or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction(PCR). It monitors the amplification of a targeted DNA molecule during the PCR(i.e.,in real time),not at its end,as in conventional PCR.<br />Two common methods for the detection of PCR products in real-time PCR are(1)non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2)sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter,which permits detection only after hybridization of the probe with its complementary sequence.




Principle

Bunyamwera Virus is a virus isolated from people who have been poisoned by ticks. May be identified as a new virus. So far, the virus is mostly tick-borne and treatable, with a low fatality rate. No cases of human-to-human transmission have been reported. Bunyavirus is naturally infected in many vertebrates and arthropods (mosquitoes, ticks, sandflies, etc.), can infect mice, and can grow in cell cultures of some mammals, birds, and mosquitoes. In humans, it can cause diseases similar to influenza or dengue fever, haemorrhagic fevers (Lifter Valley fever, Crimean-Congo haemorrhagic fever, etc.) and encephalitis (California encephalitis). There are three transmission types: mosquito, tick and phlebotomus.
Primers and probes in this experiment have been optimized with high sensitivity. Provide positive controls to distinguish false negative samples. With high specificity, the primers are designed according to the highly conserved region of the novel Bunyavirus and will not cross-react with RNA of other viruses.

Applications

It is only suitable for scientific research, not for clinical diagnosis.

Procedure

1. Sample processing and preparation of PCR reaction system.
2. Add the amplification template, cover the PCR reaction cover, mix well, centrifuge at low speed instantaneously, and transfer to the PCR instrument.
3. Set the program for PCR amplification.
4. Data analysis.

Materials

• Sample Type: serum