Analysis of Alkaline Phosphatase (Human) by ELISA (CAT#: STEM-MB-0655-LGZ)

Introduction

Alkaline phosphatase (ALP or AKP) is an enzyme that is widely distributed in human liver, bone, intestine, kidney, placenta and other tissues and is excreted from the liver to the bile. This enzyme catalyzes the removal of the 5 '-phosphate group from the nucleic acid molecule, thus converting the 5' -P end of a DNA or RNA fragment to the 5 '-OH end. Clinical determination of ALP is mainly used in the diagnosis and differential diagnosis of skeletal and hepatobiliary diseases, especially the differential diagnosis of jaundice. For unexplained high ALP serum levels, isoenzymes can be measured to help identify their organ origin.

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Principle Applications Procedure Materials Notes Related Products

Principle

Enzyme-linked immunosorbent assay (ELISA) is an enzyme-labeled solid phase immunoassay technique. Its basic principle is to bind the antigen (or antibody) to the solid phase carrier, and the antigen (or antibody) and a certain enzyme link to enzyme labeled antigen (or antibody). During detection, the sample to be tested and the enzymic antigen (or antibody) react with the antigen (or antibody) on the solid phase carrier according to certain procedures, and then remove the unreacted part by washing method. After adding the substrate, the substrate is catalyzed by the enzyme on the solid phase carrier to produce colored substances. Through qualitative or quantitative detection of the amount of colored products, the content of the substance to be measured in the sample can be determined.

Applications

Bone Disorders

Procedure

1. Add standards or samples to each well and incubate.
2. Pour off the liquid in the well, biotinylated antibody working solution and incubate.
3. Add enzyme conjugate working solution and incubate.
4. Add substrate TMB and incubate.
5. Add stop solution and measure OD value.
6. Calculation of results.

Materials

• Sample Type: Other biological fluids, Plasma, Serum, Tissue Homogenate

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